Explore Tumblr blogs with no restrictions, modern de and the best experience. The increasing trend of obesity is one of the most major public health concern and health related issues . According to an epidemiologic study conducted in countries around the world in1. The scope of the outbreak obesity is varying substantially between nations, largest rise in Oceania and the lowest trend was estimated in India .
In the USA, 34 percent of adults aged above 20 suffered from obesity in A review study indicated that obesity reached an alarming level in all ages of the East Mediterranean countries. In the same vein, a research of systemic review has conducted a study regarding the effect of BMI on cardiovascular diseases which indicated that each 5 unit raised in BMI increases the chances of heart problems by 29 percent .
Homocysteine is considered to be associated with microalbuminuria, which is a serious indicator of the risk factor of future cardiovascular disease. Homocysteine is also known to mediate heart attack, whenever its level raises, the risk of arterial artery problems such as atherosclerosis would increase [5,6]. It is a phosphor containing amino acid which is formed during methionine metabolism . Epidemiology studies revealed that high levels of homocysteine in blood plasma could be a risk factor for cardiovascular, heart attack and peripheral vascular diseases and it causes atherosclerosis through three ways of intra-arterial wall damage, interference in the blood-clotting factors, and the oxidation of low density lipoprotein .
The prevalence of hyper homo cysteinemia is estimated to be about 5 percent in the general population and 13 to 14 percent among patients with symptoms of atherosclerosis.
However, these estimates are based on a cut above the 90th or 95th percentile of total homocysteine distribution in the general population [7,9]. Various surveys have shown that levels of this amino mass are at a high level in obese or overweight as well as those who do not have regular exercise. Yet, there is debate on the effects of physical activity on this factor .
The effect of stair climbing with moderate intensity exercise on cardio-respiratory Fitness, blood lipids, and serum homocysteine were examined among sedentary young women. This study showed that these exercises can favorably make some changes in homocysteine cardiovascular risk factors and blood lipids profiles of inactive young women .
Moreover, no change in homocysteine level had been reported among 6 inactive men who participated in a walking activity with low intensity exercise . In addition, another study showed that homocysteine concentration does not lead to ificant changes in sub maximal exercise.
Hence, the training intensity, sex and age are the factors affecting the above index . Nevertheless, level of participation and different levels of interest in continuing exercise are the most important keys to reduce weight in overweight population although it may be exhausting. Therefore, doing sport activity such as Pilates can have a prominent role in increasing their interest in effect Table 1.
A collection of specialized sport activities affecting body and mind by using special equipment is called Pilates. It can increase power or endurance of whole body and even target the deepest muscles. Pilates exercise is named after its founder, Joseph Pilates, who developed a series of exercises in the s to encourage physical and mental conditioning . Strength, more body balanced, flexibility and core stability are emphasized in Pilates exercise to control of posture, movement, and breathing . A survey in this domain showed that Pilates exercise among untrained women for 8 weeks le to decreased serum creatine kinase, LDL, TG and Cholesterol.
They also reported that Pilates increases HDL among these people . Moreover, it has been investigated the effect of 24 weeks of Pilates exercise among elderly women and reported that this activity culminates in increased bone strength and decreased body lipid mass .
Nonetheless, there is no conclusive result about the effect of Pilates on homocysteine level and it has not been clearly identified whether the intensity employed in Pilates exercises has any effect on the amount of homocysteine of overweight women. Hence, the mass research deed to investigate the effect of 8 weeks of Pilates exercise on protein and mRNA level of serum homocysteine and BMI among overweight young women Table 2. The volunteers had the required features for participation in this project, including; no cardiovascular disease, not consuming special medicine, no smoking, not having a regular exercise.
Physiologic characteristics of volunteers including height, weight, age, BMI, heart rate, systolic and diastolic blood pressure were recorded. Participations were well informed about the study prior to the experiment and written consent was obtained from them. A double-blind study method was applied, and the participants were randomly ased into 2 groups of 10; 1 control and 2 Pilates exercise groups. Pilates effect group performed Pilates exercise 3 times per week for 8 weeks. It contains movements which engaged abdominal muscles, hips, waist, legs and shoulder belt and it was performed on a mat without any specific equipment in three conditions of sitting, standing and lying down.
During this period, the control group was also barred from participating in regular physical activity.
Prior the exercise protocol and at the end of eighth weeks, BMI was recorded, and blood sample was collected to measure protein or mRNA level of homocysteine. All procedures involving experiments were carried out in strict accordance of the United States Institute of Research guidelines and approved by the Medical Centre Board of Tehran University Table 3. The body mass index was calculated by measuring height in meters, weight in kilograms and putting them in the following formula:.
Blood samples were collected at 24 hours before the pilates exercise and 24 hours after the last exercise session of 8 weeks training in fasting state through the elbow antecubital vein of all subjects for measurement of homocysteine mRNA and protein expressions. The level of serum homocysteine protein was measured by homocysteine measurement Elisa kit Axis-shield diagonistmade in Germany.
A set of standard serial dilutions of known concentrations of homocysteine were provided by the manufacturer and were used to construct a standard curve in order to determine the homocysteine levels. Homocysteine which was attached to protein was changed to free homocysteine and then it was changed to S-adenosyl L homocysteine Figure 1.
This method was able to detect small differences between samples compared to other methods .
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All amplification experiments were done in 3 biological replicates. Denaturation and annealing steps were performed for 40 cycles.
In this study, the Kolmogorov - Smirnov was used for the normal distribution of data as well as t-test paired for comparing pre-test and post-test stages within each group. Independent t-test was used for between-group comparison of indices in pre- and post-test stages.
All data in ificance level of This study reports a ificant change in homocysteine level among overweight women after eight weeks of Pilates exercise.
A study investigated the effect of aerobic exercise on homocysteine level among overweight women which suffered from polycystic ovary syndrome showed that a 6-week aerobic exercise caused a ificant decrease in the level of homocysteine . In addition, similar study reported findings in young untrained women . On the other hand, Di Santolo et al, investigated the relationship between recreational sports and homocysteine level among young women and found no ificant change in the level of homocysteine .
The effect of two types of exercise on homocysteine level have been examined, as well as other cardiovascular diseases risk factors.
It was revealed that a week aerobic and resistive exercise does not make any ificant change on the level of homocysteine, lipid profile and maximal aerobic power . Furthermore, Bambaeichi et al, investigated the effect of increasing aerobic exercise on homocysteine level in young men and reported that the level of homocysteine did not have a ificant change in the experimental group .
Various factors such as; intensity, type, or length of activity, gender or age of the participants have been considered to be effective on the divergent findings of the researchers. However, the effect of exercise on homocysteine can be explored from different aspects. During doing physical activity or recovery, amino acids more likely play an important role in anaplerotic functions sustaining the whole metabolic apparatus, as well as synthesis of other proteins, such as carnitine or melatonine.
Increasing the energyreleasing reactions in the muscles; more induction in catabolism of amino acids such as methionine would be increasing. Also, regular sport activities increase renovation and repair muscle tissue by metabolic reactions require. Since methionine is an amino utilized in protein formation which starting material for numerous biochemical molecules. There is this possibility that Pilates assists in decreasing homocysteine level and changing homocysteine to methionine le to prevents its effect in the blood through increasing absorption of vitamins which are effective in homocysteine cycle especially group B vitamins which decrease homocysteine during its menatbolosm .
In addition, reduction of oxidative stress indices of regular exercise can also reduce levels of homocysteine . Also, it is likely that insulin has an impact on homocysteine metabolism and its level through affecting activities of CBS, MTHFR enzymes, Systationine Gamma-lyase and betaine homocysteine methyltransferase .
Moreover, it seems that sport activities affect insulin role in decreasing homocysteine by reducing CRP and resistance to insulin . The relationship between body composition and homocysteine is mass of great importance. Although in the present study no ificant relationship was found between them, BMI totally decreased with homocysteine reduction in Pilates group.
The relationship between body composition and homocysteine have been investigated among Korean men and women in aged of 30 to 55 which had high homocysteine level. Findings of their study revealed that increase in total body fat content and decrease in LBM have a ificant relationship with increased homocysteine. They reported that decrease in LBM is a suitable index for predicting homocysteine increase in different people . Our findings showed that 8 weeks of Pilates exercise causes a ificant decrease in BMI level among young overweight women.
However, this change was not considered ificant in the control group. In this domain, some consistent and contradictory studies can be mentioned. A 4 week of Pilates exercise on body composition have assessed in young girls. They showed that BMI level, waist size and blood pressure ificantly decreased after 4 weeks of Pilates exercise .
They declared that BMI level and waist-to-hip ratio ificantly decreased compared to pre-test . Alternatively, 5-week Pilates exercise on trunk power, endurance and flexibility among low active adult women had no any ificant change in BMI level . Furthermore, no ificant change also caused after doing 8 weeks Pilates exercise on fitness indices among low-active women . It seems as if the amount of subcutaneous fat, exercise intensity and the amount of nutrition control of the participants are among the influential factors with contradictory findings.
Different factors can be pointed out regarding the effective mechanisms on Pilates exercise. A negative relationship was reported between body composition and the level of VO2max . It appears that sport activity can be effective in decreasing suitable BMI through increasing Lipoprotein Lipaz enzyme, fat tissue, blood flow, active muscles flow, and expanding the activity of hormones mass in metabolism . In the similar vein, it has been reported a ificant increase in aerobic power as a result of 8 weeks of Pilates exercise among obese women . In conclusion, findings of this study showed that eight weeks of aerobic exercise ificantly decreased the amount of homocysteine serum and body mass index in overweight young women.
However, despite BMI level changes caused by Pilates, it seems that BMI decrease is not the major and effective factor on homocysteine decrease among overweight women. Probably there are effect factors in this domain which have not been measured in this research.
It is considered as the limitation of this study and should be taken into consideration in future researches. The most common pediatric leukemia is acute lymphoblastic leukemia ALL.